Briefly, the GP–PCR reaction was carried out using 50 ?l of PCR solution containing 10 m M Tris HCL pH 8.3, 50 m M KCl, dos00 ? M of each deoxynucleotide, 3.5 m M of MgCl2, 1 U of DNA polymerase (AmpliTaq; Perkin-Elmer, USA) and 25 pmol of each of the GP5+ and biotinylated GP6+ primers (Eurogentec, Belgium): 40 cycles of amplification were carried out using a Perkin-Elmer 9600,USA thermocycler. Lanjutkan membaca “HPV-DNA identification is did by the a basic GP5+/GP6+ PCR dependent assay, due to the fact explained by de Roda Husman et al (1995a)”
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HPV-DNA identification is did by the a basic GP5+/GP6+ PCR dependent assay, due to the fact explained by de Roda Husman et al (1995a)